Anion Carriers of Mitochondrial Membranes [Paperback]

In order to provide a functional organisation of the various biochemical processes, the intracellular environment is subdivided into compartments formed by organelles. Mitochondria serve to restore energy-rich compounds, while energy is mainly consumed in the cytosolic space. Molecules involved in these metabolic cycles have to cross the mitochondrial membrane via specialized carriers embedded in the inner mitochondrial membrane. All relevant structural and functional aspects of the Anion Carriers of Mitochondrial Membranes as well as isolation procedures, reconstitution experiments and biogenesis of the carriers, kinetics of transport as well as the metabolic regulation mechanisms are treated in this volume.In order to provide a functional organisation of the various biochemical processes, the intracellular environment is subdivided into compartments formed by organelles. Mitochondria serve to restore energy-rich compounds, while energy is mainly consumed in the cytosolic space. Molecules involved in these metabolic cycles have to cross the mitochondrial membrane via specialized carriers embedded in the inner mitochondrial membrane. All relevant structural and functional aspects of the Anion Carriers of Mitochondrial Membranes as well as isolation procedures, reconstitution experiments and biogenesis of the carriers, kinetics of transport as well as the metabolic regulation mechanisms are treated in this volume.I. Isolation and Reconstitution of Carriers.- Purification and Characterization of Three Mitochondrial Substrate Carriers: the Phosphate, the 2-Oxoglutarate and the Dicarboxylate Carriers.- Purification and Reconstitution of the 2-Oxoglutarate Carrier from Bovine Heart and Liver Mitochondria.- Hydroxyapatite Chromatography as a Tool for the Isolation of Anion Carriers and Other Membrane Proteins.- Purification of the Monocarboxylate Carrier by Affinity Chromatography.- Recent DevellÍ