Antibiotics Methods and Protocols [Paperback]

This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies.  Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research.

Part I: Production and Design

1. Antibiotics - Precious Goods in Changing Times

Peter Sass

2. Mining Bacterial Genomes for Secondary Metabolite Gene Clusters

Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert

3. Production of Antimicrobial Compounds by Fermentation

Henrik Harms, Gabriele M. K?nig and Till F. Sch?berle

4. Structure Elucidation of Antibiotics by NMR Spectroscopy

Georgios Daletos, Elena Ancheeva, Raha Orfali, Victor Wray, and Peter Proksch

5. Computer-Aided Drug Design Methods

Wenbo Yu and Alexander D. MacKerell, Jr.

6. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents

Alexander Zipperer and Dorothee Kretschmer

Part II: Mode of Action

7. Application of a Bacillus subtilis Whole-cell Biosensor (P