Antibody Engineering Methods and Protocols [Hardcover]

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques.  The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Part I: In Silico Methods

 

1. Antibody Design and Humanization via In Silico Modeling

            Vinodh B. Kurella and Reddy Gali

 

2. Antibody Affinity Maturation by Computational Design

            Daisuke Kuroda and Kouhei Tsumoto

 

3. Use of IMGT? Databases and Tools for Antibody Engineering and Humanization

            Marie-Paule Lefranc, Fran?ois Ehrenmann?, Sofia Kossida, V?ronique Giudicelli, and Patrice Duroux