Antibody Engineering [Paperback]

As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition,Antibody Engineeringis the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts.Antibody Engineering, Second Edition--written by leading experts and now thoroughly updated--is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry.

1. Introduction: Recombinant Antibodies: Future Trends
2. Human Monoclonal Antibodies from V-Gene Repertoiries Expressed on Bacteriophage
3. Synthetic Antibodies
4. Engineering the Antibody Combining Site by Codon-Based Mutagenesis in a Filamentous Phage Display System
5. Human Metaphoric Antibodies from a Phagemid Library
6. Strategies for Humanizing Antibodies
7. Single-Chain Fv Design and Production by Preparative Folding
8. Expressing Antibodies in Escherichia Coli
9. Vector and Approaches for the Eukaryotic Expression of Antibodies and Antibody Fusion Proteins
10. The Cloning of Hybridoma V Regions for their Ectopic Expression in Intracellular and Intercellular Immunisation
11. The Expression of 'Single Chain' Antibodies in Transgenic Plants