Antibody Engineering A Practical Approach [Paperback]

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies.

1. Construction and use of antibody gene repertoires
2. Affinity maturation of antibodies using phage display
3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs
4. Measuring antibody affinities in solution
5. Measuring antibody affinities using Bio-sensors
6. Analysis of human antibody sequences
7. Rodent to human antibodies by CDR grafting
8. Rodent to human antibodies by phage display
9. Choosing and manipulating effector functions
10. Secretion and purification of antibody fragments expressed in bacteria
11. Production of single chain Fv monomers and multimers
12. Expression of immunoglobulin genes in mammalian cells
13. In vitro linkage of antibody fragments into dimeric and multimeric forms

This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions. --Doody's Health Sciences Book Review Home Page