Antimicrobial Peptides Methods and Protocols [Hardcover]

This volume provides basic and advanced laboratory protocols for the non-specialist and the experienced researcher. Chapters are divided into three parts covering synthesis and characterization of AMPs, studying the interaction of AMPs with model systems or bacteria, and assaying selected biological activities of AMPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Part I: Properties, Design, Synthesis and Characterization of AMPs

1. Antimicrobial Peptides: An Introduction

Evan F. Haney, Sarah C. Mansour, and Robert E.W. Hancock

 

2. Tools for Designing Amphipathic Helical Antimicrobial Peptides

Davor Jureti, Damir Vuki1evi, and Alessandro Tossi

 

3. Chemical Synthesis of Antimicrobial Peptides

Lena M?nzker, Alberto Oddo and Paul R. Hansen

 

4. Microwave-Assisted Synthesis of Antimicrobial Peptides

Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, and Thavendran Govender

 

5. High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides

Mojtaba Bagheri and Robert E. W. Hancock

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