Arthritis Research Methods and Protocols [Paperback]

Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.

1. Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction

Junichi Kikuta and Masaru Ishii

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2. Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients

??????????? Shingo Eikawa, Shusaku Mizukami, and Heiichiro Udono

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3. Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors

??????????? Takeshi Kameyama and Akinori Takaoka

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4. Principled for the Use of in vivo Transgene Techniques: Overview and lƒ