Basic Cloning Procedures [Paperback]

Juris Steinbergs, Aleksander Tsimanis: cDNA Synthesis and Cloning.- Eriks Jankevics: DNA Sequencing. - Elita Avota, Normunds Licis: RNA in Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- Janis Klovins, Valdis Berzins: PCR-Based Site-Specific Mutagenesis.- Indrikis Muiznieks, Nils Rostoks: Analysis of Specific Protein--DNA Interactions.- Illar Pata, Erkki Truve: Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- Irina Sominskaya, Kaspars Tars: Immunological Methods for Analysis of Recombinant Proteins.- Tiina Tamm, Erkki Truve: Protein Synthesis in Cell-Free Systems.This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.Basic laboratory techniques, approved in several international molecular biology courses
Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information
The protocols are written by authors with intensive practical experience
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.Springer Book ArchivesDE