Basic Exercises in Immunochemistry A Laboratory Manual [Paperback]

1 Isolations and Preparations.- Exercise No. 1 Fractionation of Serum Proteins by Ammonium Sulfate.- Exercise No. 2 Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Column.- Exercise No. 3 Isolation of IgG by DEAE Cellulose.- A Column Chromatography.- B Batch-Type Operation.- Exercise No. 4 Dissociation of Macroglobulins With 2-Mercaptoethanol.- A.- B.- Exercise No. 5 Enzymatic Cleavage of IgG..- Exercise No. 6 Chromatographic Separation of the IgG Fragments.- Exercise No. 7 Separation and Isolation of H and L Chains of IgG.- Exercise No. 8 Affinity Chromatography and Its Application to Antibody Isolation.- Exercise No. 9 Cellulose Acetate Paper Electrophoresis of Human Serum.- Exercise No. 10 Disc Electrophoresis in Polyacrylamide Gel.- A Separation of Serum Proteins.- B SDS Gel Electrophoresis of Erythrocyte Membrane Proteins.- c Molecular Weight Determination by SDS Gel Electrophoresis.- Exercise No. 11 Separation of Human Hemoglobins by Electro-focusing.- Exercise No. 12 Conjugation of Antibodies With Fluorescein and Rhodamine.- A Fluorescein.- B Rodamine.- Exercise No. 13 Enzymatic Labeling of Proteins With 125I.- A.- B.- Exercise No. 14 Preparation of Peroxidase-Labeled Antibody.- Exercise No. 15 Preparation of Soluble Dinitrophenyl Proteins.- Exercise No. 16 Preparation of Dinitrophenyl- and Trinitrophenyl-Labeled Erythrocytes.- A.- B.- Exercise No. 17 Isolation of Cell Membranes From Ascites Tumors.- Exercise No. 18 Preparation and Use of Synthetic Liposomes.- Exercise No. 19 Isolation of Murine Transplantation Antigen Preparation.- Exercise No. 20 Isolation of Bacterial H-Antigens.- Exercise No. 21 Extraction of Bacterial 0 Antigens (Endotoxins or Lipopolysaccharides).- A Trichloroacetic Acid Method.- B Phenol-Water Procedure.- c Chloroform-Methanol Extraction of Endotoxic Glycolipid (EGL) From Rough Mutants.- Exercise No. 22 Preparation of 14C-Labeled Endotoxin and Endotoxic Glycolipid (EGL).- Exercise No. 23 Isolation of Bacterial Capsularlc)