Bioanalytical Chemistry [Hardcover]

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry

• Provides an all in one approach for both beginners and experts, from a broad range of backgrounds, covering introductions, theory, advanced concepts and diverse applications for each method

• Each chapter progresses from basic concepts to applications involving real samples

• Includes three new chapters on Biomimetic Materials, Lab-on-Chip, and Analytical Methods
• Contains end-of-chapter problems and an appendix with selected answers

Preface to Second Edition xix

Preface to First Edition xxi

Acknowledgments xxiii

1. Quantitative Instrumental Measurements 1

1.1. Introduction 1

1.2. Optical Measurements 2

1.2.1. UV-Visible Absorbance 3

1.2.2. Turbidimetry (Light-Scattering) 5

1.2.3. Fluorescence 5

1.2.4. Chemiluminescence and Bioluminescence 7

1.3. Electrochemical Measurements 8

1.3.1. Potentiometry 10

1.3.2. Amperometry 10

1.3.3. Impedimetry 11

1.4. Radiochemical Measurements 12

1.4.1. Scintillation Counting 12

1.4.2. Geiger Counting 12

1.5. Surface Plasmon Resonance 13

1.6. Calorimetry 14

1.6.1. Differential Scanning Calorimetry (DSC) 15

1.6.2. Isothermal Titration Calorimetry (ITC) 16

1.7. Automation: Microplates, Multiwell Liquid Dispensers and Microplate Readers 16

1.8. Calibration of Instrumental Measurements 18

1.8.1. External Standards 18