The second edition of Biological Aging:? Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Biological Aging:? Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.
This book details recent breakthroughs in technology that have served to advance the study of aging. It features step-by-step, readily reproducible laboratory protocols as well as key tips on troubleshooting and avoiding known pitfalls.
1. Cell Senescence Culturing Methods
Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol
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2. Digital Image Analysis of Cells Stained with the Senescence-Associated Beta-Galactosidase Assay
Liran I. Shlush and Sara Selig
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3. Analysis of Biomarkers of Caloric Restriction in Aging Cells
Yuanyuan Li and Trygve O. Tollefsbol
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4. Cell Sorting of Young and Senescent Cells
Graeme Hewitt, Thomas von Zglinicki, and Jo?o F. Passos
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5. Studying the Replicative Life Span of Yeast Cells
David A. Sil]
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