Bioluminescence Methods and Protocols [Paperback]

This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a usefulcomplement to the first and second edition for new and experienced researchersalike.

 

 Label-free Cell Phenotypic Identificationof D-Luciferin as an Agonist for GPR35.- Synthetic BioluminescentCoelenterazine Derivatives.- Molecular Cloning of SecretedLuciferases from Marine Planktonic Copepods.-  How to Fabricate FunctionalArtificial Luciferases for Bioassays.- QuantumYield Determination Based on Photon Number Measurement, Protocols for FireflyBioluminescence Reactions.- Bioluminescent Ligand-ReceptorBinding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of AminopeptidaseN in vitro and in vivo.-  Firefly Luciferase-basedSequential Bioluminescence Resonance Energy Transfer (BRET)-FluorescenceResonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Changewith a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast andMammalian Cells.-&nbspl3