This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough,
Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.
Dedication&
Preface&
Table of Contents&
Contributing Authors&
1. Engineering Citric-Acid Based Porous Scaffolds for Bone Regeneration
Jacqueline J. Masehi-Lano and Eun Ji Chung
2. Multifunctional Self-Assembling Peptide-Based Micelles for Targeted Intracellular Delivery: Design, Physicochemical Characterization, and Biological Assessment
Yejiao Shi, Ran Lin, Honggang Cui, and Helena S. Azevedo
3. Electrospinning Functionalized Polymers for Use as Tissue Engineering Scaffolds
Lesley W. Chow
4. Low-lƒ-