Bone Marrow Processing and Purging a Practical Guide [Hardcover]

Bone Marrow Processing and Purging: A Practical Guide provides an up-to-date practical guide to the major ex vivo procedures associated with bone marrow transplantation. Previously, this information was communicated primarily by word of mouth; now experts in the field present detailed descriptions and evaluations of methods for marrow harvesting, evaluation (including tumor infiltration, flow cytometric analysis, and colony assays), comparative methods for automated nucleated cell separation and enumeration, tumor cell purging, T cell depletion, stem cell selection, gene transfer, and cytopreservation. Special sections address quality control and FDA regulations.

The book provides a unique information source intended for clinicians, researchers, technical staff, transplant nurses, and medical students involved in this rapidly expanding area of medicine.INTRODUCTION AND GENERAL GUIDELINES. Preface (Adrian P. Gee). An Introduction to Bone Marrow Transplantation and Processing (A. John Barrett). Regulatory Aspects of Bone Marrow Processing and Purging (Curtis L. Scribner). Quality Control in Bone Marrow Processing (Adrian P. Gee). HARVESTING, SHIPPING, AND NUCLEATED CELL SEPARATION. Bone Marrow Harvesting and Reinfusion (Jennifer G. Treleaven). Transportation of Bone Marrow for In Vitro Processing and Transplantation (William E. Janssen and Carlos Lee). Nucleated Cell Separation Using the Fenwall CS-3000' (Herbert M. Cullis, Ellen Areman, and Charles S. Carter). Use of the Cobe 2991' Cell Processor for Bone Marrow Processing (John D. McMannis). Bone Marrow Processing With the Haemonetics V50 Plus' (MaryLynne Hartl). BONE MARROW EVALUATION. Bone Marrow Cell Counting: Methodological Issues (Elizabeth J. Read, Charles S. Carter, and Herbert M. Cullis). Detection of Metastatic Tumor Cells in Bone Marrow (Thomas J. Moss). Flow Cytometric Analysis of Human Bone Marrow (James G. Bender and Dennis Van Epps). Quantitative Assays for Human Hemopoietic Progenitor ClÃj