Brain Energy Metabolism [Hardcover]

Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab.

Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.

1. Determination of CO₂ Production in Subcellular Preparations like Synaptosomes and Isolated Mitochondria Using ¹⁴C-Labeled Substrates and Radioactive CO₂ Measurements

??????????? Mary C. McKenna and Irene B. Hopkins

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2. Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membrane by Heterologous Protein Expression in Xenopus Oocytes

??????????? Holger M. Becker

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3. Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells

??????????? Ketki Tulpule, Michaela C. Hohnholt, Johannes HirrlilÓÙ