This detailed volume compiles state-of-the-art methodologies for the study of brassinosteroid hormones, contributed by recognized researchers in the field, in order to bring together different experimental and theoretical biology techniques for addressing the questions of how brassinosteroids function in Arabidopsis and other agriculturally valuable species, such as rice and sorghum. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Brassinosteroids: Methods and Protocols assesses a wide range of appealing methodologies for experts as well as researchers with a general interest in hormone signaling in plants.
1. Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
Danuae Tarkowsk? and Miroslav Strnad
2. Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Johan M. Winne, Niloufer G. Irani, Jos Van den Begin, and Annemieke Madder
3. Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
Hongning Tong and Chengcai Chu
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