Cancer Cell Signaling Methods and Protocols [Hardcover]

Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study.?Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment.

Part I: Targeting Cancer Cell Metabolism as Anticancer Therapy

1. Use of Biguanides to Improve Response to Chemotherapy

??????????? Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner

2. Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival

??????????? Sang Hyeok Woo, Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner

3. Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy

??????????? Nariman Ansari, Stefanie M?ller, Katharina H?tte, Stefanie Rakel, Patrick Antonietti, Donat K?gel, Ernst H.K. Stelzer, and Francesco Pampaloni

Part II: Epigenetic Control of Canl“,