This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.?
Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study.?
1. Introduction to Cancer Stem Cells: Past, Present, and Future
??????????? David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi, Chitra Venugopal, and Shiela K. Singh
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2. Surface Markers for the Identification of Cancer Stem Cells
??????????? Vinod Gopalan, Farhadul Islam, and Alfred King-yin Lam
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3. The Role of CD44 and Cancer Stem Cells
??????????? Liang Wang, Xiangsheng Zuo, Keping Xie, and Daoyan Wei
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4. Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
??????????? Luigi Mele, Davide Liccardo, and Virginia Tirino
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5. Isolation of Cancer Stem Cells by Side Population Method
??????????? Masayuki Shimoda, Masahide Ota, al%
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