Capillary Electrophoresis Methods and Potentials [Paperback]

1 Introduction.- 2 Principles of Capillary Electrophoresis.- 3 Theoretical Foundations and Their Influence on the Analytical Results.- 3.1 Electrophoretic Migration.- 3.2 Conductivity.- 3.3 Electroosmotic Flow.- 3.4 Band Broadening.- 3.4.1 Efficiency losses through diffusion.- 3.4.2 Efficiency loss through temperature effects.- 3.4.3 Loss in efficiency through electrodispersion.- 3.4.4 Efficiency losses through wall adsorption.- 3.4.5 Efficiency losses through overloading of the separation system.- 3.4.6 Efficiency losses through superimposition of flow profiles.- 3.4.7 Summary.- 4 Instrumentation.- 4.1 Power Supply.- 4.2 Capillaries.- 4.3 Sample Introduction.- 4.3.1 Pressure injection.- 4.3.2 Hydrostatic injection.- 4.3.3 Electrokinetic injection.- 4.3.4 Sample-split systems.- 4.3.5 Enrichment effects in sample introduction: sample stacking.- 4.4 Thermostating.- 4.5 Detection.- 4.5.1 UV detection.- 4.5.2 Fluorescence detection.- 4.5.3 Conductivity detection.- 4.5.4 Other detection methods.- 4.5.5 Derivatization reactions.- 4.6 Special Problems of Quantitative Analysis in CE.- 5 Capillary Zone Electrophoresis (CZE).- 5.1 Principles of Optimization in CZE.- 5.1.1 Effect of pH.- 5.1.2 Effect of buffer concentration.- 5.1.3 Buffer selection.- 5.1.4 Applications.- 5.2 Indirect Detection Methods in CE.- 5.2.1 Principles of indirect detection techniques.- 5.2.2 Separation of cations with indirect UV detection.- 5.2.3 Separation of anions with indirect UV detection.- 5.2.4 Analysis of cations and anions with indirect fluorescence detection.- 5.3 Capillary Zone Electrophoresis of Proteins.- 5.3.1 Separations in uncoated capillaries.- 5.3.1.1 Selection of the pH.- 5.3.1.2 Addition of salts to the buffer.- 5.3.1.3 Use of buffer additives for the separation of proteins.- 5.3.1.4 Dynamic coating of capillaries.- 5.3.2 Protein separations with surface-modified capillaries.- 5.3.2.1 Coatings for capillary electrophoresis.- 5.3.3 Overview of important chemical coatings for proteinl£¡