Capillary Electrophoresis of Proteins and Peptides Methods and Protocols [Hardcover]

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)-Fluorescence Analysis of Proteins

 Isabelle Le Potier, Audrey Boutonnet, Vincent Ecochard, and Fran?ois Couderc

 

2. Discrimination of Glycoproteins from Unglycosylated Proteins in Capillary Electrophoresis: Two-Color LIF Detection Coupled with Post-Column Derivatization

  Takashi Kaneta

 

3. Capillary Zone Electrophoresis-Mass Spectrometry of Intact Proteins

  Elena Dom?nguez-Vega, Rob Haselberg, and Govert W. Sol/