Cardiac Gene Therapy Methods and Protocols [Hardcover]

This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Part I: Overview

 

1. Current Methods in Cardiac Gene Therapy: Overview

            Kiyotake Ishikawa and Roger J. Hajjar

 

Part II: Genetic Modification

 

2. Silencing Genes in the Heart

            Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller