Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
1.????? ?Second Generation Codon Optimized Minicircle (Comic) For Non-Viral Reprogramming Of Human Adult Fibroblasts
Sebastian Diecke, Leszek Lisowski, Nicholas M. Mordwinkin, Nigel G. Kooreman, and Joseph C. Wu
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2.????? ?Scalable Cardiac Differentiation Of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates
Celine Bauwens and Mark Ungrin
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3.????? ?Preparation And Characterization Of Circulating Angiogenic Cells For Tissue Engineering Applications
Aleksandra Ostojic, Suzanne Crowe, Brian Mcneill, Marc Ruel, and Erik J. Suuronen
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4.????? Isolation And Expansion Of C-Kit Positive Cardiac Progenitor Cells By Magnetic Cell Sorting
Kristin M. French and Michael E. Davis
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5.????? Synthesis Of Aliphatic Polyester Hydrogel For Cardiac Tissue Engineering
Sanjiv Dhingra, Richard D. Weisel, and Ren-Ke Li&ll³n