c-di-GMP Signaling Methods and Protocols [Hardcover]

This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling.?The chapters, divided into eight major parts, guide readers through methods on synthesis, detection, quantitation, modulation of the levels of c-di-GMP present in cells, procedures to detect and evaluate the interaction of c-di-GMP, and up and coming approaches focusing on the inhibition of c-di-GMP signaling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

?Authoritative and cutting-edge, c-di-GMP Signaling: Methods and Protocols aims to inspire researchers to try new approaches.

1. Discovery of the Intracellular Signaling Molecule Cyclic di-GMP

Ute R?mling and Michael Y. Galperin

PARt I: Synthesis of c-di-GMP

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2. Enzymatic Production of C-di-GMP Using a Thermophilic Diguanylate Cyclase

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3. Synthesis of [³²P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations

Barbara I. Kazmierczak

Part II: Detection and Quantitation of c-di-GMP

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4. High-performance Liquid Chromatography (HPLC)-based Detection and Quantitation of Cellular c-di-GMP

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