Cell Biology Assays Proteins [Paperback]

Protein assay methods are used for protein identification with blood groups, cell surface markers, drugs and toxins. This text features comprehensive protocols essential for researchers studying various areas of biological and medical sciences. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a vareity of technologies and model systems.

• Focus on protein identification using mass spectrometry
• Step-by-step procedures detailing materials, procedures, comment and pitfalls
• Information on the plethora of technologies needed to tackle complex problems

1. Protein Determination
2. Phosphopeptide Mapping: A Basic Protocol
3. Coupling of Fluorescent Tags to Proteins
4. Radioiodination of Proteins and Peptides
5. Free-Flow Electrophoresis
6. Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.
7. High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis
8. Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples
9. Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood Plasma
10. Image Analysis and Quantitation
11. Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
12. Autoradiography and Fluorography: Film-Based Technil£*