Cell Cycle - Materials and Methods [Paperback]

During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual.
A glossary helps the reader to cope with the complex cell cycle terminology.During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual.
A glossary helps the reader to cope with the complex cell cycle terminology.One Techniques for Studying the Mammalian Cell Cycle.- 1 Use of Immunohistochemistry in Cell Cycle Studies.- 2 Immunofluorescence Techniques in Cell Cycle Studies.- 3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes.- 4 Techniques for Monitoring Cell Cycle Phases.- 5 Centrifugal Elutriation.- 6 Synchronization of Mammalian Cells.- 7 Microinjection and Electroporation of Macromolecules into Live Cells.- 8 Stable and Transient Transfections in Cell Cycle Studies.- 9 Quantification of Premature and Normal Mitosis.- Two Techniques for Studying the Xenopus Cell Cycle.- 10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies.- Three Techniques to Study the Yeast Cell Cycle.- 11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe.- 12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe.- Four Techniques for Studying the Cell Cycle of Any Species.- 13 Identificatil6