Cell Imaging Techniques Methods and Protocols [Hardcover]

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters? covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

?Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.

This contribution to the Methods in Molecular Biology collection has been updated to include new chapters covering colocalization and laser microdissection, among other emerging techniques. It features the series trademark of clear and reproducible protocols.

1.? Digital Images are Data  and Should be Treated as Such

Douglas W. Cromey

?2. Epi-fluorescence Microscopy

Donna J. Webb and Claire M. Brown

3. Live-Cell Migration and Adhesion Turnover Assays

Lacoste, J. Young, K. and Brown, C. M.

4. Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in HulC<