Cell Imaging Techniques [Paperback]

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.Molecular Beacons: Fluorescent Probes for Detection of Endogenous mRNAs in Living CellsDiana P. BratuSecond-Harmonic Imaging of CollagenGuy Cox and Eleanor KableVisualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent MicroscopyMichael Wm. Roe, Jerome F. Fiekers, Louis H. Philipson, and Vytautas P. BindokasMultifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung TissueMaria Stern, Douglas J. Taatjes, and Brooke T. MossmanEvaluation of Confocal Microscoplc+