Cell Migration Methods and Protocols [Hardcover]

This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.

Preface&

Table of Contents&

Contributing Authors&

1. Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories

Irene Dang and Alexis Gautreau

Nicolas Molinie and Alexis Gautreau

3. An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition Aleksandra S. Chikina and Antonina Y. Alexandrova

4. An In Vitro System to Study the Epithelial-Mesenchymal Transition In Vitro Natalya A. Glushankova, Svetlana N. Rubtsova, and Irina Y. Zhitniak

5. Detection of Migrasomes

Yang Chen, Ying Li, Liang Ma, and Li Yu

6. 3D Endothelial Cell Migration