Cell'Cell Interactions Methods and Protocols [Paperback]

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport MeasurementsMichael Aschner, Vanessa A. Fitsanakis, Ana Paula Marreilha dos Santos, Luisa Olivi, and Joseph P. BresslerInteractions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional ConsequencesGeraldine Canny, Alexander Swidsinski, and Beth A. McCormickThe Role of Junctional Adhesion Molecules in Interactions Between Vascular CellsTriantafyllos Chavakis and Valeria OrlovaCell-Cell Interactions on Solid MatricesNancy lc+