Cellular Heterogeneity Methods and Protocols [Hardcover]

This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.?

Part I: General

1. Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level

??????????? Natasha S. Barteneva and Ivan A. Vorobjev

2. Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies

??????????? Albert Gough, Tong Ying Shun, D. Lansing Taylor, and Mark Schurdak

3. Image-Based Tracking of Heterogeneous Single Cell Phenotypes

??????????? Katherin Patsch, Shannon M. Mumenthaler, and Daniel Ruderman

Part II: Cytometry in Cellular Heterogeneity Research

4. High-Dimensional Single Cell Analysis with Mass Cytometry

??????????? Tess Melinda Bl³r