Cellulases Methods and Protocols [Hardcover]

This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.

Preface&

Table of Contents&

Contributing Authors&

Part I Introduction Chapter and a Case Example

1.Cellulases: Role in Lignocellulosic Biomass Utilization

Sanjeev Kumar Soni, Amita Sharma, and Raman Soni

2.Discovery of a Novel Fungus with an Extraordinary b-Glucosidase

Peter Stephensen L?beck and Mette L?beck

Part II Protocols 

Discovery of New Cellulases Using Classical or Molecular Methods

3.  Isolation and Screening of Cellulolytic Filamentous Fungi

Mette L?beck and Peter Stephensen L?beck

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