Cereal Genomics Methods and Protocols [Hardcover]

In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.? Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.? Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered.?Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

This excellent new resource in Springers Methods in Molecular Biology series has expert guidance on todays techniques for analyzing and manipulating cereal genes. It includes instructions for isolating DNA and RNA from both vegetative tissue and seeds.

?1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing

Agnelo Furtado

2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)

Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa

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3. RNA Extraction from Cereal Vegetative Tissue

Julie A. Pattemore

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4. RNA Extraction from Developing or Mature Wheat Seeds

Agnelo Furtado

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5. cDNA Library Preparation

Maarten Kooiker and Gang-Ping Xue