Chemical Proteomics Methods and Protocols [Paperback]

The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function. ?In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

Chemical Proteomics provides key techniques to investigate chemical proteomics. The text presents analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.

Part I: Introduction

1. Mass Spectrometry-Based Chemoproteomic Approaches

Marcus Bantscheff

2. Chemical Proteomics in Drug Discovery

Gerard Drewes

Part II: Small Molecules and Probe Design

3. Compound Immobilization and Drug-Affinity Chromatography

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

4. Affinity-based Chemoproteomics with Small Molecule-peptide Conjugates

Chaitanya Saxena