Chromosome Architecture Methods and Protocols [Hardcover]

This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture. Chromosome Architecture:Methods and Protocols guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

New Advances in Chromosome Architecture.- Single MoleculeNarrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose SignalTransduction Of Live Yeast Cells.- Single-Molecule Imaging to Characterise the TransportMechanism of the Nuclear Pore Complex.- Using Fluorescence Recovery after Photobleaching (FRAP)To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex InVivo.- Imaging DNA Structure by Atomic Force Microscopy.- Investigating Bacterial ChromosomeArchitecture Using Epifluorescence Microscopy.- Transverse Magnetic TweezersAllowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA.- Probing Chromosome Dynamics in Bacillus Subtilis.- Systems Biology Approaches for Understanding Genome Architecture.- InVivo And