Chronic Myeloid Leukemia Methods and Protocols [Hardcover]

This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Molecular Detection of BCR-ABL in Chronic Myeloid Leukemia

Ya-Zhen Qin and Xiao-Jun Huang

2. Induction of Chronic Myeloid Leukemia in Mice

Haojian Zhang and Shaoguang Li

3. CML Mouse Model Generated from Leukemia Stem Cells

Yiguo Hu

4. Immunological Analyses of Leukemia Stem Cells

Kazuhito Naka and Yoshihiro Takihara

5. Cell Cycle Analysis of CML Stem Cells using Hoechst33342 and Propidium Iodide

Ngoc DeSouza, Megan Zhou, and Yi Shan

6. Histological and In Vivo Microscopic Analysis of the Bone Marrow Microenvironment in a Murine Model of Chronic Myelogenous Leukemia

Eva S. Weissenberger and Daniela S. Krause

7. The Culture Repopulation Ability (CRA) Assay and Incubation in Low Oxygen to Test Anti-Leukemic Drugs on Imatinib-resistant CML Stem-like Cells

Giulia Cheloni and  Michele Tanturli

8. Tumor Suppressor Analysis in CML

Oliver Herrmann and Mirle Schemionek

9. Detecting Autophagy and Autophagy Flux in Chronic MyelolG