Circular RNAs Methods and Protocols [Hardcover]

This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.? Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field.?

1. Detection and Reconstruction of Circular RNAs from Transcriptomic Data

Yi Zheng and Fangqing Zhao

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2. Deep Computational Circular RNA Analytics from RNA-seq Data

Tobias Jakobi and Christoph Dieterich

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Daphne A. Cooper, Mariela Cort?s-L?pez, and Pedro Miura

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4. Analysis of Circular RNAs using the Web Tool CircInteractome

Amaresh C. Panda, Dawood B. Dudekula, Kotb Abdelmohsen, and Myriam Gorospe

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5. Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR

Andreas W. Heum?ller and Jes-Niels Boeckel

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6. Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization

Anne Zirkel and Argyris Papantonis

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7. Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as

Christine Kocks, Anastalc&