This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more.?Written?in the highly successful?Methods in Molecular Biology?series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circula
ting Tumor Cells: Methods and Protocols?is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.
Dedication&
Acknowledgement&
Foreword&
Preface&
Table of Contents&
Contributing Authors&
?
1.?????? Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer Origin
Chian-Hui Lai and Ying-Chih Chang
2.?????? Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability
Emily S. Park, Simon P. Duffy, and Hongshen Ma
3.?????? A Novlcl
![Circulating Tumor Cells Methods and Protocols [Hardcover]](/itemImage/2/5/4/2/2_774149.jpg)