Cryopreservation and Freeze-Drying Protocols [Paperback]

This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.

PART I: Fundamental Aspects And Freezing Technology?????????????????????????????????????????????

1?????? Principles of Cryopreservation???????????????????????????????????????????????????????????????????

David E. Pegg

2????????? Principles of Cryopreservation By Vitrification ??????????????????????????????????????????????????????? Gregory M. Fahy and Brian Wowk

3????????? Modeling and Optimization of Cryopreservation ????????????????????????????? ??????????? ??????????? James Benson

4????????? The Principles of Freeze-Drying ??????????????????????????????????????????????????????????????????????????????? Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward

PART II: Methods To Study Freezing and Drying Processes?????????????????????????????????????