Crystallography in Molecular Biology [Paperback]

1 Crystallization and Data Collection.- Crystallization of integral membrane proteins.- Crystallization of protein and nucleic acids: A survey of methods and importance of the purity of the macromolecules.- Synchrotron radiation and macromolecular crystallography.- Data collection.- 2 Phase Problem.- Density modification methods.- Anomalous scattering in macromolecular structure analysis.- Single molecule electron crystallography.- Structure analysis of bacteriorhodopsin by electron crystallography.- Neutron diffraction: contribution to high and low resolution crystallography.- 3 Graphics and Structure Analysis.- Electron density fitting with computer graphics: A review and a glimpse.- Computer grapnics in the study of macromolecular interactions.- Prediction of protein structure from amino acid sequence.- 4 Water and Dynamics.- X-ray analysis of polypeptide hormones at ? 1? resolution: Anisotropic thermal motion and secondary structure of pancreatic polypeptide and deamino-oxytocin.- Water at biomolecule interfaces.- Tho water structure in 27n insulin crystals.- The structure of water around a macrocyclic receptor: A Monte Carlo study of the hydration of 18-crown-6 in different conformations.- Simulating protein dynamics in Solution : Bovine pancreatic trypsin inhibitor.- 5 Drug Design  Site Directed Mutagenesis.- Rational design of DNA minor groove-binding anti-tumor drugs.- Protein crystallography and drug design.- Enzyme mechanism : What X-ray crystallography can(not) tell us.- The role of separate domains and of individual ami no-acids in enzyme catalysis, studied by site-directed mutagenesis.- Selected and directed mutants of T4 phage lysozyme.- 6 Viruses.- The structure of a human common cold virus (rhinovirus 14) and its functional relations to other picornaviruses.- The structure of poliovirus at 2.9 ? Resolution: Crystallographic methods and biological implications.- The structure of cowpea mosaic virus at 3.5 ? Resolution.- Adenovirus architecture.- 7 Prlã-