Detection of Blotted Proteins Methods and Protocols [Hardcover]

This detailed volume provides methods and techniques for detection after blotting.? Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.

1. Western Blotting: Origin and Ascent of the Species

W. Neal Burnette

?

2. Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic Evaluation

J.P. Dean Goldring

?

3. Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose

J.P. Dean Goldring

?

4. Detection of Blotted Proteins: Not all Blockers are Created Equal

Vishal Kothari and Suresh T. Mathews

?

5. Protein Stains to Detect Antigen on Membranes

Anil Dsouza and Hal Scofield

?

6. Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting

F. Javier Alba and Salvador Bartolom?

?

7. Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment ComlsŒ