Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in thePractical Approachseries. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
1. Screening expression libraries with antibody and protein probes 2. Expression of foreign proteins inE.coliusing plasmid vectors and purification of specific polyclonal antibodies 3. Purification of over-produced proteins fromE.colicells 4. Production of monoclonal antibodies against proteins expressed inE.coli 5. Expression of cloned genes in yeast 6. Intraction trap cloning with yeast 7. The baculovirus expression system
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