Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of? PCR amplicons obtained from DNA genomic markers with different? inheritance patterns. In?DNA Electrophoresis Protocols for Forensic Genetics,?expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It? includes protocols for? profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and? mtDNA? hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful? Methods in Molecular Biology ?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
This volume in the Methods in Molecular Biology series covers methods in forensic DNA profiling, including protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and? mtDNA? hypervariable regions HV1 and HV2.
Part I: Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments??
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1.???????? An Overview of? DNA Typing Methods for Human Identification: Past, Present, and Future
Robyn Thompson, Silvia Zoppis, and Bruce McCord
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2.???????? Capillary Electrophoresis and 5-channel LIF Detection of a 26plex Autosomal STR Assay for Human Identification
Carolyn R. Hill.
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3.???????? Capillary Electrophoresis of Mini-STR Markers to Genotype Highly Degraded DNA Samples
Michael Coble&lĂ5