DNA Replication Methods and Protocols [Hardcover]

Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments.?As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.

1. A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells

Nikko P. Torres and Grant W. Brown

?

2. Microscopy Techniques to Examine DNA Replication in Fission Yeast

Marc D. Green, Sarah A. Sabatinos, and Susan L. Forsburg

?

3. High-Resolution Analysis of Mammalian DNA Replication Units

Vadim O. Chagin, Marius Reinhart, and M. Cristina Cardoso

?

4. Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing

Marta Bialic, Vincent Coulon, Marjorie Drac, Thierry Gostan, and Etienne Schwob

?

5. Measuring DNA Content by Flow Cytometry in Fission Yeast

Sarah A. Sabatinos and Susan L. Forsburg

?

6. Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast

Nicholas Rhind