DNA Sequencing Protocols [Paperback]

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.DNA SEQUENCING PROTOCOLS, Second EditionEdited by Colin A. Graham and Alison J. M. HillContents1. Introduction to DNA sequencingColin A. Graham and Alison J. M. Hill2. M13 cloningJoachim Messing3. M13 sequencingQingzhong Yu4. Primer Design and Primer directed sequencingUlrike Gerischer and Peter D?rre5. Direct sequencing of DNA produced in a Polymerase Chain ReactioUlrike Gerischer6. Solid phase fluorescent sequencing of the CFTR geneHarry Cuppens and Jean-Jacques Cassiman7. l3+