DNA Sequencing Protocols [Paperback]

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.Introduction to DNA Sequencing, Colin A. Graham and Alison J. M. Hill. The Universal Primers and the Shotgun DNA Sequencing Method, Joachim Messing. M13 Sequencing, Qingzhong Yu. Primer Design and Primer-Directed Sequencing, Ulrike Gerischer and Peter D?rre. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction, Ulrike Gerischer. Solid Phase Fluorescent Sequencing of the CFTR Gene, Harry Cuppens and Jean-Jacques Cassiman. Shotgun DNA Sequencing, Alan T. Bankier. Cycle Sequencing, Robert W. Blakesley. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers, Johannes Zschocke and Georg F. Hoffmann. Automated Fluorescent DNA Sequencing on the ABI PRISM 377, Jonathan R. E. MacBeath, Suzanna S. Harvey, and Nicola J. Oldroyd. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer, David Watts and Jonathan R. E. MacBeath. Fluorescent Sequencing Protocols for the ALF, Shirley McQuaid. Fluorescent Sequencing for Heterozygote Mutation Detection, Colin A. Graham and Alison J. M. Hill. Sequence Databases and the Internet, Anne E. Hughes. DNA Sequencing by Capillary Array Electrophoresis, Norman J. Dovichi and JianZhong Zhang. Index. DNA Sequencing Protocollı