DNA Vaccines Methods and Protocols [Hardcover]

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.? Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.? Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.

Part I: DNA Vaccine Design and Enhancement

?

1. A Blueprint for DNA Vaccines Design

??????????? Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi

?

2. Enhancement of Plasmid-Mediated Transgene Expression

??????????? Daniela Fioretti, Sandra Iurescia, and Monica Rinaldi

?

3. Strategies for Improving DNA Vaccines Performance

??????????? Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi

?

4. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies

??????????? Elisabete Borges Freitas, Ana Margarida Henriques, Miguel Fevereiro, Duarte Miguel Prazeres, and Gabriel Amaro Monteiro

?

5. Progresses in DNA-Based Heterologous Prime-Boost ImmulÓb