DNA Viruses Methods and Protocols [Paperback]

A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.Part I. Viral DetectionViral DetectionFeng Wang-Johanning and Gary L. JohanningQuantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1Servi J. C. Stevens, Sandra A. W. M. Verkuijlen, and Jaap M. MiddeldorpProfiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCRServi J. C. Stevens, Antoinette A. T. P. Brink, and Jaap M. MiddeldorpQuantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In VivoDonna R. Hochberg and David A. Thorley-LawsonDetection and Quantification of the Rare Latently Infected Cell Undergoils3