DNA'Protein Interactions Principles and Protocols [Paperback]

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.Filter-Binding Assays
Peter G. Stockley

Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
Marc-Andr? Laniel, Alain B?liveau, and Sylvain L. Gu?rin

DNase I Footprinting, Beno?t Leblanc and Tom Moss. Footprinting with Exonuclease III
Willi Metzger and Hermann Heumann

Hydroxyl Radical Footprinting
Evgeny Zaychikov, Peter Schickor, Ludmilla Denissova, and Hermann Heumann

The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA
Brenda F. Kahl and Marvin R. Paule

Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10 -Phenanthroline-Copper
Athanasios G. Papavassilou

Uranyl Photofootprinting
Peter E. Nielsen

Osmium Tetroxide Modification and the Study of DNA-Protein Interactions
James A. McClellan

Determination of a Transcription Factor-Binding Site by Nuclease Prol“8