Eicosanoid Protocols [Paperback]

Use of a Retroviral Vector for Expression of a Foreign Gene, Brent W. Miller and Aubrey R. Morrison. Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues, William S. Powell. Assessment of Cyclooxygenase RNA Expression by Northern Hybridization, Zhonghong Guan and Aubrey R. Morrison. Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin, ShaAvhree Y. Buckman, Alane T. Koki, Dorothy A. Edwards, and Alice P. Pentland. Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography, Mukut Sharma, Beatrice Hucke, and Elias A. Lianos. Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry, M. Kerry O'Banion and John A. Olschowka. Assessment of Cyclooxygenase Protein Expression by Western Blotting, Zhonghong Guan and Aubrey R. Morrison. Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells,Alfred N. Fonteh. Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction, Alfred N. Fonteh, William Reed, and James M. Samet. Enzyme Immunoassays (EIA) of Eicosanoids, Kingsley Yin and Lloyd J. Forman. Transcellular Regulation of Eicosanoid Biosynthesis, Karsten Gronert, Clary B. Clish, Mario Romano, and Charles N. Serhan. Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry,Samuel C. Blackman, Catherine Borg, David C. Yeomans,and Guy C. Le Breton. Epoxyeicosatrienoic Acids: Biosynthesis, Regulation, and Actions, John D. Imig. Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts,Srinivasa T. Reddy and Harvey R. Herschman. Assessment of 5-Lipoxygenase Activity and Cellular Distribution, Robert A. Lepley. Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids, Eric G. Spokas, Joshua Rokach, and Patrick Y.-K. Wong. Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibitionlc)