Electroporation and Sonoporation in Developmental Biology [Paperback]

In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.

Electroporation.- Short History of Electroporation for the Study of Developmental Biology.- In Ovo Electroporation as a Useful Tool to Pursue Molecular Mechanisms of Neural Development in Chick Embryos.- Applications of Tol2 Transposon-Mediated Gene Transfer for Stable Integration and Conditional Expression of Electroporated Genes in Chicken Embryos.- Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systems in Vertebrate Embryos.- In Ovo Electroporation for Targeting the Somitic Mesoderm.- Method of Electroporation for the Early Chick Embryo.- Enhancer Analysis.- Electroporation of Nucleic Acids into Chick Endoderm Both In Vitro and In Ovo.- Electroporation into the Limb: Beyond Misexpression.- Retinal Fiber Tracing by In Ovo Electroporation.- Retroviral Vector-Mediated Gene Transfer into the Chick Optic Vesicle by In Ovo Electroporation.- Clonal and Widespread Gene Transfer by Proviral Electroporation for Analysis of Brain Laminar Formation.- Electroporation into Cultured Mammalian Embryos.- In Utero Electroporation: Assay lB